Hyalocytes were FACS isolated from five enucleated eyes immediately after enucleation as well as from nine patients undergoing vitrectomy due to macular hole or macular pucker. Standard RNA-sequencing was applied for transcriptome analysis. Details regarding the isolation and sequencing protocol are provided in Boneva et al. (2020).
Fastq files of all replicates can be downloaded from the Gene Expression Omnibus (GEO) database using the following links. Please cite one of the publications shown above, when using data for your research.
| Sample | Age at surgery (years) | Sex | FFPE/unfixed | Download from GEO |
|---|---|---|---|---|
| 1 (vitrectomy) | 72.0 | male | unfixed | GSE147657 |
| 2 (vitrectomy) | 72.8 | male | unfixed | GSE147657 |
| 3 (vitrectomy) | 87.2 | male | unfixed | GSE147657 |
| 4 (vitrectomy) | 77.3 | female | unfixed | GSE147657 |
| 5 (vitrectomy) | 69.0 | male | unfixed | GSE147657 |
| 6 (vitrectomy) | 78.0 | female | unfixed | GSE147657 |
| 7 (vitrectomy) | 72.1 | female | unfixed | GSE147657 |
| 8 (vitrectomy) | 76.1 | female | unfixed | GSE147657 |
| 9 (vitrectomy) | 67.1 | male | unfixed | GSE147657 |
| 10 (enucleation) | 52.7 | female | unfixed | GSE180705 |
| 11 (enucleation) | 51.2 | male | unfixed | GSE180705 |
| 12 (enucleation) | 92.5 | female | unfixed | GSE180705 |
| 13 (enucleation) | 55.0 | male | unfixed | GSE180705 |
| 14 (enucleation) | 60.5 | male | unfixed | GSE180705 |